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Amino acid mutations in the H-site can significantly alter the catalytical activity of GST enzymes towards their substrates [88, 89]

Ethical considerations also play a role

A predicted site in selenoprotein P (SELENOP, Figure 2C) is at position 56, 3 residues prior to the first selenocysteine, such that cleavage here would likely interfere with the N-terminal redox activity, possibly without affecting the Se-transport function of the rest of the protein

For those without contraindications of vitamin B12, the potential benefits of vitamin B12 supplementation are often deemed to outweigh the associated risks