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The list of differentially expressed genes was uploaded to Ingenuity Pathway Analysis (QIAGEN IPA), and core analysis yielded a list of canonical pathways, of which the top 10 upregulated and downregulated pathways were visualized on bar plots using ggplot v3.5.0 [29]

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The mechanism is the same: ethanol metabolites disrupt hypothalamic GHRH release and increase somatostatin activity regardless of the delivery vehicle