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When you take hydrolyzed collagen peptides, the amino acids (primarily glycine, proline, and hydroxyproline) are absorbed and accumulate in cartilage tissue, where they appear to stimulate chondrocytes to produce more collagen and reduce cartilage breakdown markers

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Protein concentrations were determined using a BCA assay (Beyotime, P0009)

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If the research protocol can tolerate this level of uncertainty, use the peptide in non-critical pilot work rather than in formal study cohorts