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T-cells also use cytokines cells as messenger molecules which aid cell to cell communication for ramping up its response to move cells towards sites of inflammation, infection and trauma

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Tyramide signal amplification was performed using SuperBoost kit (Invitrogen, B40935 for Alexa Fluor 596 and B40932 for Alexa Fluor 488) according to the manufacturers instructions and applied to the section for 10 min at room temperature

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