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We propose an innovative artificial intelligence (AI) method to generate whole-brain high spatial resolution NWU maps derived from NCCT alone, presenting a promising neuroimaging biomarker for early ischemia visualization and prediction of lesion reversibility

Western blotting (WB), immunohistochemistry (IHC) and Immunofluorescence (IF) are adopted to investigate the hub targets expression and signaling pathway activation (e.g., PI3K-AKT pathway, estrogen receptor pathway)

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More refined studies (ATAC-seq, ChIP-seq H3K27ac) map activated enhancers and involved transcription factors (CREB, MEF2)

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