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Indeed, during APAP-induced toxicity there is an increased generation of mitochondrial superoxide anion production, stimulated by impaired mitochondrial respiration and formation of adducts between the reactive metabolite NAPQI and sulfhydryls moieties from mitochondrial proteins (Yan et al., 2010)

To be able to measure the production of ROS, studies often use flow cytometry in combination with fluorescence or chemiluminescent assays to detect levels of commonly produced ROS, such as hydrogen peroxide, a hydroxyl radical, and a peroxynitrite anion [60]

As responsible citizens, it is time we recognize the gravity of clinical research

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