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Pickarts original discovery grew out of experiments in which the tripeptide influenced the survival and growth of liver cells, and later synthesis work compiled reports that GHK could protect hepatic tissue in experimental poisoning models

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In terms of specific regulatory effects, the protective role of mitophagy is mainly reflected in a state of moderate activation: when cells are subjected to mild stress and mitochondria undergo local damage, mitophagy can specifically recognize and clear damaged, depolarized mitochondria, effectively reducing the abnormal release of ROS and the cytoplasmic leakage of mtDNA

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Others maintain benefits with less frequent treatments