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The slides were coverslipped with DPX mounting medium (Sigma, St

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The elution was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column that was pre-equilibrated with 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 1 M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS

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Some of the symptoms include: tingling or numbness in the hands, feet or legs cognitive difficulties or memory loss a swollen, inflamed tongue difficulty walking/balance issues anaemia depression weakness fatigue The only way to know for sure is through a blood test