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The cell lysates (1ml) were mixed 1:1 with 85% sucrose solution, layered in the bottom of the centrifuge tube (Ultra clear Beckman centrifuge tubes) and overlaid sequentially with 6ml 30% and 3.5ml 5% sucrose solution to make a non-continuous sucrose gradient

Figure 3 In conclusion, SLC7A11 has a pro-survival effect, and SLC7A11-mediated cystine uptake can help cells re-establish redox homeostasis in response to oxidative stress, whereas inhibition of SLC7A11 can lead to depletion of cellular GSH and thus make the cells more sensitive to chemotherapy or radiotherapy

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& Jarvi, K