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3.2 CB-839 exposure leads to mitochondrial ROS accumulation in CLL cells Considering that glutathione is the main antioxidant that provides protection from excessive ROS production, we evaluated the consequences of glutathione synthesis inhibition on cellular ROS for 4 hours and mitochondrial SOX accumulation for 24 hours after incubation with CB-839 ( Figures 1E, G )

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Measurements of light response curve and CO 2 response curve Light and CO 2 response curves were measured using a LI-6400 Portable Photosynthesis System (Li-Cor Inc., Lincoln, NE, USA) on the middle fully developed leaves of cucumber seedling after 4 days of treatments and 4 days of recovery