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The mixture was vortexed for 30 s, ultrasonicated in an ice bath for 5 min, incubated at -20C for 1 h, and centrifuged at 12,000 rpm (4C) for 15 min

Community sources describe the calculation in blunt terms: researchers apply enterprise-grade quality assurance to the $300 peptide and skip verification entirely on the solvent that peptide depends on

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