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Briefly, to verify the targeted binding between miR-29b-3p and the GGCT gene, a 2000-bp DNA fragment (derived from the transcription start site of the GGCT gene) was inserted into the pmir-GLO-Basic vector to construct the wild-type pmir-GLO-GGCT-3 UTR reporter plasmid (wt) and the mutant pmir-GLO-GGCT-3 UTR reporter plasmid (mut)

In another embodiment the buffer is a sodium citrate buffer having a pH of about 5.5 to about 7.0

10 [online] Available at: Pogribna, M., Melnyk, S., Pogibny, I., Chango, A., Yi, P

You can also think of GSH as the active, working form of the molecule

However, this is not necessary with L-carnitine either