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Quantitative real-time polymerase chain reaction (qRT-PCR) analysis Cellular RNA was extracted from mouse kidney tissues and HK-2 cells

General Procedure for Peptide Coupling Synthesis of (S,S) Di-Tert-Butyl (5-(Tert-Butoxy)-4-((Tert-Butoxycarbonyl)Amino)-5-Oxopentanoyl)Glutamate 1 ( S )Boc-Glu-O t Bu (1 mmol, 303.4 mg), ( S )HCl,H-Glu(O t Bu)-O t Bu (1 mmol, 295.8 mg), BOP (1.2 mmol, 530.7 mg), and DIEA (3 mmol, 0.52 ml, pH = 8.5) were dissolved in THF (15 ml) and stirred at room temperature

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Renal fibrosis: novel insights into mechanisms and therapeutic targets