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When endothelial cells release nitric oxide, nearby smooth muscle cells in the blood vessel wall respond by relaxing

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Meanwhile, in the functional tests, we observed that the tubular formation ability of endothelial cells was significantly decreased after OGD treatment (Figure 3E)

These suspensions were incubated at 37C for 60 minutes, either immediately after adding hydroxyurea or after a 20-hour incubation period