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2.13 Dot blotting analysis Proteins from 5 L of bile samples were transferred to PVDF membrane (Merck Millipore, Shanghai, China) using the Bio-Dot microfiltration apparatus according to the manufacturers instructions (Bio-Rad Laboratories, Inc., United States)

Briefly, under visualization with an operating microscope, 50 l of any one of the formulations described herein and varying concentrations of P407 are administered to the animals