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Encephalopathy is caused by unconjugated bilirubin has the ability to pass through the blood-brain barrier, entering the central nervous system

Cells were then detached by gentle scraping with a cell scraper and analyzed by Accuri-C6 flow cytometry using FL1 channel (BD biosciences, USA)

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This allows p53 to trigger the apoptotic pathway, removing senescent cells while leaving healthy, functional cells unharmed