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They were then spun down, removed of their supernatant, and resuspended and washed with several changes of PBS, then washed over night in 5 mL PBS

Most of these formulations contain ethinyl estradiol (EE) as the synthetic estrogen in combination with various progestins

However, the effect may be transient unless underlying deficiencies are present

Hydrogels: Hydrogels have excellent biocompatibility and drug loading capacity, and can be used to achieve sustained and controlled release of drugs