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Mass and purity are verified vacuum sensation is not a validated quality metric

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Conditioned medium was harvested, filtered through 0.45-m filters, concentrated by ultracentrifugation (81550 g, 2 h at 4 C) and stored at 80 C

Centrifuge at 1000 rpm for 5 min (at speed), using slow acceleration (400 rpm.min-1) and no brake during deceleration

Rather than focusing only on NAD + in isolation, its important to understand where it fits within the complex system and how it can be optimized in the context of improving performance of the whole system