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After vigorous vortexing, this solution was centrifuged (7000 g for 5 min) and then the bottom layer was collected for spectroscopic analysis and diluted in HCl 3M/MeOH 1:3

It may influence sleep-related signaling, circadian rhythms, and stress pathways in experimental models, though findings are limited to preclinical and in vitro studies

This endogenous activation provides sustained cellular protection compared to direct free radical scavenging by exogenous antioxidants

Funding This study is supported by the University of Zagreb, Zagreb, Croatia, grant number 099

Influence of beta-blockers on melatonin release