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Clark DW, Palle K (2016) Aldehyde dehydrogenases in cancer stem cells: potential as therapeutic targets

This architecture creates an extraordinarily high binding affinity (pK = 16.44), roughly 10 million times stronger than other tripeptides and comparable to albumins copper binding

PLoS One 7 (3), e33172

Subsequently, the amphiphilic drug dissolved in the external aqueous phase can penetrate into the entire phospholipid bilayer and then interact with the captured agent to lock the drug inside the liposome

When the temperature was lowered to 20 C, the mixture was centrifuged at 10,000 g for 20 min, after which absorbance values were measured at 600, 450, and 532 nm