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Firstly all three strains, the GDH2 and GDH3 deletion mutants and the wild-type strain, grown in MM medium were compared to investigate the metabolic differences between mutant and wild-type strains during the morphological transition ( gdh2 -MM vs WT-MM, gdh3 -MM vs WT-MM)

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Screening of selected LAB genomes for GSH system genes We looked for eight major genes involved in glutathione synthesis, transport and metabolism in fully sequenced genomes of selected food grade LAB and other organisms of probiotic and dairy importance namely bifidobacteria and propionibacteria