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Aquino, Universidad Austral, Argentina Reviewed by Moon Nyeo Park, Kyung Hee University, Republic of Korea Sohail Mumtaz, Gachon University, Republic of Korea Updates Copyright 2025 Zhao, Chen, Qiao, Wang, Ali, Li and Yin

Key findings include: Upregulation of Shh pathway components in ischemic brain regions Enhanced neural stem cell proliferation and differentiation Improved angiogenesis supporting tissue repair Modulation of blood-brain barrier integrity The Shh pathway activation appears particularly relevant in stroke recovery models, where enhanced neurogenesis and vascular support correlate with functional improvements

Cell suspensions were serially diluted and plated on TSA with 0.4% activated charcoal (Sigma) to neutralize residual antibiotics, allowing enumeration of surviving bacteria

Reconstitute with 2 mL bacteriostatic water