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After centrifugation, the cells were resuspended in PBS with 2 mM EDTA-0.5% BSA (MACS running buffer) in accordance with the manufacturer's instruction (Miltenyi Biotec)

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Peptide degradation pathways Tirzepatide is a 39-amino-acid peptide with specific structural modifications that make it effective, including alpha-aminoisobutyric acid (Aib) substitutions at positions 2 and 13 (which protect against DPP-4 enzyme degradation in the body) and a C20 fatty diacid chain at position 20 (which enables albumin binding for a longer half-life)

6-Phosphogluconate dehydrogenase links cytosolic carbohydrate metabolism to protein secretion via modulation of glutathione levels