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Following the addition of 6 ml acetone containing BHT (0.1 %, w/v), the homogenates were thoroughly mixed using a Ohaus Multi-Tube Vortex mixer, for 5 min at 2,500 rpm, allowed to stand for 5 min, and centrifuged at 2,200 g for 5 min at 4 C

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In addition, beta-diversity for both IF groups was altered significantly by time ( p = 0.001), with no significant differences between groups