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Human trials also help identify side effects

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They found that the 2D motility of HT-1080 fibrosarcoma cells, MDA-MB-231 breast cancer cells, and normal breast epithelial MCF-10 A cells is faster on fast- (~ 100 s) relative to slow-relaxing (~ 2,000 s), soft (2 kPa) substrates [143]

Their protective functions include reducing paracellular permeability, boosting innate immune responses against pathogens, and consolidating the physical barrier provided by the mucous layer (163)