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The arginine substitution at position 3 and the N-terminal extension collectively reduce IGFBP affinity by approximately 1001,000-fold in preclinical binding assays

BPC-157 therapy initiated immediately post-injury produced consistent healing effects at all time points, as confirmed by macroscopic/microscopic assessment, ultrasound, magnetic resonance imaging, biomechanical testing, and functional evaluation

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Functional analysis identified copper-induced inflammatory and oxidative stress responses as key mechanisms in the WD cell model

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