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The measurement of paracellular permeability is usually quite straightforward since the movement of ions and charged organic molecules can be measured as an electrical current and the movement of neutral molecules can be measured by assaying their concentration in the bloodstream or urine or in a compartment equivalent to the subepithelial space in mounted or perfused tissue or in cultured cells grown on permeable substrates

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For research content, the safer focus is compound identity, study models, purity, COA testing, and laboratory handling

Wahab S, Anwar AI, Zainuddin AN, Hutabarat EN, Anwar AA, Kurniadi I