Growth of this strain containing the gene circuit in the presence of an inducer activates transcription of the reporter gene, producing a detectable signal, as long as the inducer exceed a specified concentration, which defines the limit of detection (LOD) or sensitivity of the circuit
Calculating concentrations incorrectly results in either underdosing or overdosing
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For instance, Exocrine Pancreatic Insufficiency (EPI), a condition where the pancreas doesn't produce sufficient digestive enzymes, can hinder the absorption of crucial nutrients, including B12
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