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Most e-cigarettes contain nicotine

Tobacco use and health Though tobacco use has reduced significantly over the years, owing largely to public health campaigns and regulations on public smoking, it still causes thousands of deaths per year

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The genomic DNA was isolated from the spermatozoa using the QIAamp DNA Mini Kit, followed by mitochondrial DNA amplification via the REPLI-g Mitochondrial DNA Kit supplied by QIAGEN (Hilden, Germany), adhering to the kits instruction manual