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To determine whether this ARE is functional to mediate NRF2-dependent upregulation of CBS gene expression, we cloned CBS promoter fragment from 1000 to +200 bp and mutant fragment with deletion of +77 to +87 bp into a pGL3-basic luciferase reporter vector, which were designated as pGL3-phCBS and pGl3-phCBS-ARE, respectively
Charlotte W
Volatile organic compounds trapped in Tenax TA were desorbed at 250C for 10 min using a thermal desorption system (TD-20, Shimadzu Co.), which was directly injected into a QP-2010 Ultra gas chromatograph/mass spectrometer (GC/MS) equipped with a Stabiliwax capillary column (60 m 0.25 mm 0.25 m, Restek, Bellefonte, PA, USA) in splitless mode
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