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Synthesis of cDNA was performed with 1 g of total RNA from each sample using Quantiscript Reverse Transcriptase, Quantiscript RT-buffer and RT Primenr-mix of QuantiTect Reverse Transcription kit (Qiagen GmbH, Hilden, Germany) according to the manufacturers protocol

Plessis SS, Page C, Franken DR (2002) Extracellular signal-regulated kinase activation involved in human sperm-zona pellucida binding

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