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37 Small sample volumes are recommended for good resolution of target protein versus other contaminants

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Total proteins were extracted with protein extraction buffer (100 mM Tris-HCl pH 8, 150 mM NaCl, 10% glycerol, 5 mM EDTA, 10 mM DTT, 2 mM PMSF, 10 mM NaF, 10 mM Na 2 MoO 4 , 2 mM NaVO 3 , 1%(v/v) NP-40, 1%(v/v) plant protease inhibitor cocktail)