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After the incubation, the cells were washed three times with PBS and mounted using Shandon Immu-Mount

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Takeaways Bacteriostatic water contains 0.9% benzyl alcohol preservative enabling multi-dose use for up to 28 days Sterile water provides maximum purity but requires single-use within 24-48 hours after opening Cell culture applications typically require sterile water due to benzyl alcohol cytotoxicity concerns Choice depends on experimental timeline, peptide sensitivity, and whether preservatives interfere with assays What is Bacteriostatic Water for Peptides