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Materials and Methods Bacterial Strains, Growth, and Stress Assays Escherichia coli TOP10 (Invitrogen) used for gene manipulation was grown on LB at 37C

Funding This work was supported by the Hungarian National Scientific Research Foundation [grant number NKFI K 125265 and NKFI FK 124871]

Integration of scRNA-seq with epigenomic approaches, such as single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) and DNA methylation profiling, allows identification of regulatory elements governing the expression of key genes, including those involved in survival, proliferation, and insulin secretion (Leenders et al., 2024

Store reconstituted TB-500 in the fridge