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In Graves disease, supplementation has been linked to the restoration of biochemical euthyroidism in certain patients, albeit data are limited

Supplementary Table 1) was mixed with 3 l of transfection reagent (DharmaFECT 1, Horizon Discovery) in a total volume of 500 l of OptiMEM (Gibco) and incubated for 20 minutes at room temperature

FIGURE 2 IGF SIGNALING IN THE CONTEXT OF LIGAND-RECEPTOR INTERACTIONS The IGF system is comprised of type I and type II receptors, their ligands (IGF-1 and IGF-2), IGFBPs, and IGFBP proteases (Le Roith et al., 2001)

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