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Results were presented as pictogram or nanogram of mediator released per milliliter of conditioned medium

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In experiments using melatonin, either melatonin (10mg/kg, Sigma) or an equivalent volume of DMSO (Sigma) was intraperitoneally injected each day for three days at 17:00, immediately after intranasal challenges had been performed

However, experts in the area generally concur that a combination of data from multiple endpoints is needed to discriminate sensitizing and/or irritant substances in vitro