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Forward primers for mutagenesis were: R38A: CTC CGC GAC TGC ATT CCA GGC GCT AAC CAA CAA GGT TTT TG R130A: CCC TTC CTC AGC CAA ACG CGG GCT TGG CAT TTT TTG Mutants were expressed and activity measured in membrane fractions as described 47

Materials and methods Animals and treatment Thirty Wistar rats were obtained from the Central Animal House of the Faculty of Medicine of Ribeiro Preto, University of So Paulo, after approval by the institution's Ethics Committee of Animal Experimentation

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However, many vitamins, such as vitamin A, vitamin B12, vitamin C, and vitamin D, are not produced by the body

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