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The supernatant and precipitate obtained were harvested by centrifugation at 14,200 g at 4 C for 10 min

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Endogenous and exogenous reactive oxygen species, such as superoxide anion, hydroxyl radical, hypohalite radical, and hydrogen peroxide, and reactive nitrogen species, such as nitric oxide, peroxynitrite, and nitrite, play a major role in the airway inflammation and are determinants of asthma severity

We thank Marisa Jakobs for the help with initial redox mobility shift assays and Robin Schumann for cloning wild-type and mutant ROGFP2