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CD8 + cytotoxic T lymphocytes, reactive to TPO and Tg, promote glandular destruction, aided by increased ICAM-1 expression on follicular cells and elevated serum levels of VCAM-1 and TIMP-1

After amino acid sequence identification and molecular docking, three peptides with the lowest binding energy were obtained: WDRPKDDGGSPIK(W1), DRGYPPVMF(W2) and SGGGGGGGLGSGGSIRSSY(W3)

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Notably, our results on GABA align with several prior studies using optimized sequences such as MEGA-PRESS [25], suggesting that methodological sensitivity is unlikely to be the sole factor responsible for the non-significant findings