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This genetic anomaly in MeCbl synthesis leads to the accumulation of homocysteine, a condition termed hyperhomocysteinemia or homocystinuria [94, 100], (Fig

Sections were washed with Tris-buffered saline (TBS) and incubated with secondary antibody anti-mouse-IgG antibody conjugated with Alexa Fluor488, anti-rabbit-IgG antibody conjugated with Alexa Fluor 594 for 2 h at room temperature in the dark