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After additional washing in PBST, nanobodies were diluted four times ten gradients starting at 400 g/mL concentration and were added to the wells and incubated for 2.5 h at 37 C

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10.1038/539179a Nature 34 HongoN.TakamuraY.NishimaruH.MatsumotoJ.TobeK.SaitoT.et al (2020)

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AOD 9604 is a synthetic peptide fragment derived from the C-terminus of human growth hormone (hGH), specifically amino acids 176191