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Cells were washed with PBS to remove excessive NEM before lysing in triton lysis buffer (50 mM TrisHCl, pH 7.5, 1 mM EDTA, 1% (v/v) Triton X-100, 150 mM NaCl) supplemented with protease inhibitor cocktail (EMD Millipore, 539134)

IV Administration Protocol: Preparation: Tourniquet for vein identification Local anesthetic for numbing the injection site Needle for initial puncture Catheter tube to maintain vein access Access cap for medication administration Syringe for medication delivery IV bags and lines for infusion Gauze, bandage, and medical tape for site protection Procedure: Inject a needle into the person's vein

These may involve: Copper deficiency , as excess zinc can block copper absorption and affect red blood cell production or immune balance

For adults, the preferred injection site is the outer aspect of the arm

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