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The release of Cu into tumor cells enhances reactive oxygen species (ROS) as well as nitric oxide (NO) production, which results in DNA fragmentation, induces pro-apoptotic protein upregulation such as Bax, and enhances caspase-3, caspase-8, and caspase-9 generation that motivates cancer cells apoptosis [68] even in solid tumor hypoxic condition [69]

287,288 In the vitro experimental of head and neck cancer 289 and triple-negative breast cancer (TNBC) 290 also verified the antitumor effects of iCRTs

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When you're studying a compound this powerful, you can't afford to have contaminants or incorrect sequences muddying your results