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An incision was made under 2% isoflurane anesthesia delivered via a nose cone, as described by Brennan et al

This phase is generally considered safe, though side effects may include diarrhea, dizziness, muscle cramps and weight gain as your body retains more water

The video explains how to inject

Thus, the dynamic interaction between microglia and astrocytes, mediated by specific cytokine and chemokine signaling pathways, critically determines neuroinflammatory trajectories and influences both degenerative and regenerative processes in CNS disorders (Jha et al., 2019

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