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In addition, they maintain the polarity of the epithelial cells by preventing the passage of molecules (lipids and proteins) from the apical membrane to the basolateral and vice versa, so the TJs have a very important function in the establishment of the intestinal barrier

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Contact mode scratching of the substrates was performed to assess whether the monolayers had the expected protein heights, using a cantilever with a bare tip and a nominal spring constant of 0.5 N/m, by applying a force ranging from a few nN for imaging to hundreds of nN for scratching as reported in Funari et al

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Diet and cataract: the Blue Mountains Eye Study