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Anaerobic bacteria grow within Candida albicans biofilms and induce biofilm formation in suspension cultures

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In addition, gnotobiotic approaches have shown that administration of Collinsella reduces the expression of tight junction proteins in enterocytes and stimulates gut leakage (Chen et al., 2016)

hNF-1 was grown and maintained as monolayer cultures at 37 C in 5% CO 2 in a humidified atmosphere, using DMEM (low glucose with l-glutamine and phenol red, Sigma-Aldrich Co., Llc.) and 5 vol% FBS (Thermo Fisher Scientific Inc.)

Although the initial damage to the brain seemed to be similar between treatment and control groups, the researchers noticed differences in how the brain degenerated over time