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For MPP3 analysis, bone-marrow cells were stained with the antibody mixture (lineage cocktail, c-Kit, Flt3 (BioLegend, 103236, 100218, 100434, 100734, 108428, 101228, 116228, 105826 and 135310), Sca1, CD48 and CD150 antibodies) for 20 min at 4 C, washed and analyzed by flow cytometry

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Similarly, there was some lack of consistency in 1 H-MRS approach and measurement, although this did not adversely affect our ability to compare across studies

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