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This dual Cas9 system takes advantage of different protospacer adjacent motif (PAM) sequences recognized by Streptococcus pyogenes Cas9 (SpCas9) versus Staphylococcus aureus Cas9 (SaCas9), enabling simultaneously knocking out two genes (Fig

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Reconstituted peptides are perishable compounds, and using degraded material introduces uncontrolled variables into research protocols