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For each qPCR reaction, 25 l of master mix was prepared by adding 12.5 l of the 2 Universal SYBR (ThermoFisher) 5.5 l of RNAse-free water, 1 l ACX primer (0.05 g /ul), 1 l TS primer (0.1 g /l) (Table 1), and 4 l of the protein sample

Current obstacles to successful local or systemic delivery of peptides include poor absorption, enzymatic degradation, and mucociliary clearance

This process contributes to improved tissue functional status, analogously to the biological principle where the removal of dysfunctional structures allows for more efficient resource utilization by healthy cells

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