NxGen-T4 DNA Ligase (Low Concentration), 1,500 U size:EACH both NTPs and dNTPs are

both NTPs and dNTPs are substrates for the enzyme

5 at 25°C)

Unit Definition: One unit of CircLigase II enzyme converts 1 pmol of a linear 5´-phosphorylated CircLigase II Standard 55-mer Oligo into an exonuclease I-resistant circular form in 1 hour at 60°C under standard assay conditions

blot-hybridization experiments

C41(DE3)pLysS

NxGen-T4 DNA Ligase (Low Concentration), 1,500 U size:EACH both NTPs and dNTPs areReliably join double stranded nucleic acid chains. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5 phosphate and 3 hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA RNA hybrids. T4 DNA Ligase Version Sizes Ligase Concentration Provided Buffer Low Concentration Ligase 1,500 U 7,500 U 2 U L 10X T4 DNA Ligase Buffer